Improvement of ligninolytic properties in the hyper lignin-degrading fungus Phanerochaete sordida YK-624 using a novel gene promoter.
Identifieur interne : 000432 ( Main/Exploration ); précédent : 000431; suivant : 000433Improvement of ligninolytic properties in the hyper lignin-degrading fungus Phanerochaete sordida YK-624 using a novel gene promoter.
Auteurs : Tatsuki Sugiura [Japon] ; Toshio Mori ; Ichiro Kamei ; Hirofumi Hirai ; Hirokazu Kawagishi ; Ryuichiro KondoSource :
- FEMS microbiology letters [ 1574-6968 ] ; 2012.
Descripteurs français
- KwdFr :
- ADN fongique (composition chimique), ADN fongique (génétique), Analyse de profil d'expression de gènes (MeSH), Analyse de séquence d'ADN (MeSH), Analyse de séquence de protéine (MeSH), Bois (microbiologie), Clonage moléculaire (MeSH), Données de séquences moléculaires (MeSH), Expression des gènes (MeSH), Gènes fongiques (MeSH), Génie métabolique (MeSH), Lignine (métabolisme), Peroxidases (biosynthèse), Phanerochaete (enzymologie), Phanerochaete (génétique), Phanerochaete (métabolisme), Protéines bactériennes (analyse), Régions promotrices (génétique) (MeSH), Électrophorèse bidimensionnelle sur gel (MeSH).
- MESH :
- analyse : Protéines bactériennes.
- biosynthèse : Peroxidases.
- composition chimique : ADN fongique.
- enzymologie : Phanerochaete.
- génétique : ADN fongique, Phanerochaete.
- microbiologie : Bois.
- métabolisme : Lignine, Phanerochaete.
- Analyse de profil d'expression de gènes, Analyse de séquence d'ADN, Analyse de séquence de protéine, Clonage moléculaire, Données de séquences moléculaires, Expression des gènes, Gènes fongiques, Génie métabolique, Régions promotrices (génétique), Électrophorèse bidimensionnelle sur gel.
English descriptors
- KwdEn :
- Bacterial Proteins (analysis), Cloning, Molecular (MeSH), DNA, Fungal (chemistry), DNA, Fungal (genetics), Electrophoresis, Gel, Two-Dimensional (MeSH), Gene Expression (MeSH), Gene Expression Profiling (MeSH), Genes, Fungal (MeSH), Lignin (metabolism), Metabolic Engineering (MeSH), Molecular Sequence Data (MeSH), Peroxidases (biosynthesis), Phanerochaete (enzymology), Phanerochaete (genetics), Phanerochaete (metabolism), Promoter Regions, Genetic (MeSH), Sequence Analysis, DNA (MeSH), Sequence Analysis, Protein (MeSH), Wood (microbiology).
- MESH :
- chemical , analysis : Bacterial Proteins.
- chemical , biosynthesis : Peroxidases.
- chemical , chemistry : DNA, Fungal.
- chemical , genetics : DNA, Fungal.
- chemical , metabolism : Lignin.
- enzymology : Phanerochaete.
- genetics : Phanerochaete.
- metabolism : Phanerochaete.
- microbiology : Wood.
- Cloning, Molecular, Electrophoresis, Gel, Two-Dimensional, Gene Expression, Gene Expression Profiling, Genes, Fungal, Metabolic Engineering, Molecular Sequence Data, Promoter Regions, Genetic, Sequence Analysis, DNA, Sequence Analysis, Protein.
Abstract
We identified a highly expressed protein (BUNA2) by two-dimensional gel electrophoresis from the hyper lignin-degrading fungus Phanerochaete sordida YK-624 under wood-rotting conditions. Partial amino acid sequences of BUNA2 were determined by LC-MS/MS analysis, and BUNA2 gene (bee2) and promoter region were PCR-cloned and sequenced. The bee2 promoter was used to drive the expression of the manganese peroxidase gene (mnp4) in P. sordida YK-624. Eighteen mnp4-expressing clones were obtained, with most showing higher ligninolytic activity and selectivity than wild-type YK-624. Examination of the ligninolytic properties of the most effective lignin-degrading transformant, BM-65, cultured on wood meal revealed that this strain exhibited higher lignin degradation and MnP activities than those of wild type. Transcriptional analysis confirmed the increased expression of recombinant mnp4 in the transformant. These results indicate that use of the bee2 promoter to drive the expression of ligninolytic enzymes may be an effective approach for improving the lignin-degrading properties of white-rot fungi.
DOI: 10.1111/j.1574-6968.2012.02556.x
PubMed: 22506973
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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<term>DNA, Fungal (genetics)</term>
<term>Electrophoresis, Gel, Two-Dimensional (MeSH)</term>
<term>Gene Expression (MeSH)</term>
<term>Gene Expression Profiling (MeSH)</term>
<term>Genes, Fungal (MeSH)</term>
<term>Lignin (metabolism)</term>
<term>Metabolic Engineering (MeSH)</term>
<term>Molecular Sequence Data (MeSH)</term>
<term>Peroxidases (biosynthesis)</term>
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<term>Phanerochaete (genetics)</term>
<term>Phanerochaete (metabolism)</term>
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<term>Sequence Analysis, DNA (MeSH)</term>
<term>Sequence Analysis, Protein (MeSH)</term>
<term>Wood (microbiology)</term>
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<keywords scheme="KwdFr" xml:lang="fr"><term>ADN fongique (composition chimique)</term>
<term>ADN fongique (génétique)</term>
<term>Analyse de profil d'expression de gènes (MeSH)</term>
<term>Analyse de séquence d'ADN (MeSH)</term>
<term>Analyse de séquence de protéine (MeSH)</term>
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<term>Données de séquences moléculaires (MeSH)</term>
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<term>Gènes fongiques (MeSH)</term>
<term>Génie métabolique (MeSH)</term>
<term>Lignine (métabolisme)</term>
<term>Peroxidases (biosynthèse)</term>
<term>Phanerochaete (enzymologie)</term>
<term>Phanerochaete (génétique)</term>
<term>Phanerochaete (métabolisme)</term>
<term>Protéines bactériennes (analyse)</term>
<term>Régions promotrices (génétique) (MeSH)</term>
<term>Électrophorèse bidimensionnelle sur gel (MeSH)</term>
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<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Lignin</term>
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<term>Molecular Sequence Data</term>
<term>Promoter Regions, Genetic</term>
<term>Sequence Analysis, DNA</term>
<term>Sequence Analysis, Protein</term>
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<term>Analyse de séquence de protéine</term>
<term>Clonage moléculaire</term>
<term>Données de séquences moléculaires</term>
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<term>Gènes fongiques</term>
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<front><div type="abstract" xml:lang="en">We identified a highly expressed protein (BUNA2) by two-dimensional gel electrophoresis from the hyper lignin-degrading fungus Phanerochaete sordida YK-624 under wood-rotting conditions. Partial amino acid sequences of BUNA2 were determined by LC-MS/MS analysis, and BUNA2 gene (bee2) and promoter region were PCR-cloned and sequenced. The bee2 promoter was used to drive the expression of the manganese peroxidase gene (mnp4) in P. sordida YK-624. Eighteen mnp4-expressing clones were obtained, with most showing higher ligninolytic activity and selectivity than wild-type YK-624. Examination of the ligninolytic properties of the most effective lignin-degrading transformant, BM-65, cultured on wood meal revealed that this strain exhibited higher lignin degradation and MnP activities than those of wild type. Transcriptional analysis confirmed the increased expression of recombinant mnp4 in the transformant. These results indicate that use of the bee2 promoter to drive the expression of ligninolytic enzymes may be an effective approach for improving the lignin-degrading properties of white-rot fungi.</div>
</front>
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<Abstract><AbstractText>We identified a highly expressed protein (BUNA2) by two-dimensional gel electrophoresis from the hyper lignin-degrading fungus Phanerochaete sordida YK-624 under wood-rotting conditions. Partial amino acid sequences of BUNA2 were determined by LC-MS/MS analysis, and BUNA2 gene (bee2) and promoter region were PCR-cloned and sequenced. The bee2 promoter was used to drive the expression of the manganese peroxidase gene (mnp4) in P. sordida YK-624. Eighteen mnp4-expressing clones were obtained, with most showing higher ligninolytic activity and selectivity than wild-type YK-624. Examination of the ligninolytic properties of the most effective lignin-degrading transformant, BM-65, cultured on wood meal revealed that this strain exhibited higher lignin degradation and MnP activities than those of wild type. Transcriptional analysis confirmed the increased expression of recombinant mnp4 in the transformant. These results indicate that use of the bee2 promoter to drive the expression of ligninolytic enzymes may be an effective approach for improving the lignin-degrading properties of white-rot fungi.</AbstractText>
<CopyrightInformation>© 2012 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved.</CopyrightInformation>
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<ForeName>Tatsuki</ForeName>
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<MeshHeading><DescriptorName UI="D020539" MajorTopicYN="N">Sequence Analysis, Protein</DescriptorName>
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<name sortKey="Kawagishi, Hirokazu" sort="Kawagishi, Hirokazu" uniqKey="Kawagishi H" first="Hirokazu" last="Kawagishi">Hirokazu Kawagishi</name>
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{{Explor lien |wiki= Bois |area= PhanerochaeteV1 |flux= Main |étape= Exploration |type= RBID |clé= pubmed:22506973 |texte= Improvement of ligninolytic properties in the hyper lignin-degrading fungus Phanerochaete sordida YK-624 using a novel gene promoter. }}
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